● Enzynomics Cytokine International unit (IU)
Description
Recombinant human glial cell line-derived neurotrophic factor (hGDNF) mature form produced in
E. coli
Synonyms
Astrocyte-derived trophic factor (ATF)
Expression Host
Escherichia coli (E. coli)
NCBI Accession Number
P39905
Amino Acid Sequence
Arg109~Ile211 with an N-terminal Methionine
N-terminal Sequence Analysis
Met-Arg-Gly-Gln-Arg
Predicted Molecular Mass
11.6 kDa (monomer); Human GDNF forms a disulfide-linked homodimer
Purity
> 97% by SDS-PAGE
Formulation
1 mg/ml human GDNF in PBS without carrier proteins
Sterility
Sterilized through a 0.22 µm membrane filter.
Biological Activity
The ED
50 as determined by the dose dependent proliferation of SH‑SY5Y human neuroblastoma cell in the presence of human GFRα-1 is 11.2~18.3 ng/ml.
Endotoxin Level
0.1 EU/μg of the protein as determined by the Limulus amebocyte lysate (LAL) method.
Shipping
The product is shipped with dry ice. Upon receipt, store it immediately at -80 °C before use.
Storage and Stability
This product can be diluted in sterile 1X PBS to at least 0.1 mg/ml in the presence of 0.1% BSA and stored for a month at 4 °C. The diluted protein can be stored in aliquots at -80 °C for long-term storage. Do not vortex and avoid repeated freeze-thaw cycles.
Figure 1. SDS-PAGE
2 μg of recombinant human GDNF was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie Brilliant Blue staining.
Figure 2. Size analysis
0.23 mg of recombinant human GDNF was was applied to gel permeation chromatography in a buffer containing 4.3 mM sodium phosphate, 1.4 mM potassium phosphate, 2.7 mM potassium chloride, and 1 M sodium chloride. Based on the calculation of molecular weight of the human GDNF (29.9 kDa), it is predicted to be a homodimer.
Figure 3. Biological Activity
The biological activity of recombinant human GDNF was determined by growth of SH‑SY5Y human neuroblastoma cell.
Figure 4 . Cell growth
Comparison of growth of SH‑SY5Y cells in the absence (A) or presence (B) of human GDNF (72 hour incubation)
