● Enzynomics Cytokine International unit (IU)
Description
Recombinant human fibroblast growth factor 9 (FGF-9) produced in
E. coli
Synonyms
Glia-activating factor (GAF), Heparin-binding growth factor 9 (HBGF-9)
Expression Host
Escherichia coli (E. coli)
NCBI Accession Number
P31371
Amino Acid Sequence
Pro3-Ser208
N-terminal Sequence Analysis
Pro-Leu-Gly-Glu-Val
Predicted Molecular Mass
23.2 kDa
Purity
> 97% by SDS-PAGE
Formulation
1 mg/ml Human FGF-9 in 50 mM Tris-HCl (pH9.2), 1 M NaCl, 10% glycerol without carrier proteins
Sterility
Sterilized through a 0.22 µm membrane filter.
Biological Activity
The ED
50 as determined by the dose-dependent proliferation of NIH-3T3 mouse fibroblast cell is 2.2 ~ 3.9 ng/ml.
Endotoxin Level
< 0.1 EU/μg of the protein as determined by the Limulus amebocyte lysate (LAL) method.
Shipping
The product is shipped with dry ice. Upon receipt, store it immediately at -80 °C before use.
Storage and Stability
This product can be diluted in sterile 1X PBS to at least 0.1 mg/ml in the presence of 0.1% BSA and stored for a month at 4 °C. Prepare aliquots and freeze at -80 °C for long-term storage. Avoid repeated freeze-thaw cycles and do not vortex.
Figure 1. SDS-PAGE
2 μg of recombinant Human FGF-9 was resolved with SDS-PAGE under reducing condition and visualized by Coomassie Brilliant Blue staining.
Figure 2. Biological ActivityThe biological activity of recombinant human FGF-9 was determined by growth of NIH-3T3 mouse fibroblast cells.
Figure 3. Size analysis
Recombinant human FGF-9 was applied to gel permeation chromatography in 50 mM Tris-HCl (pH9.2), 1 M NaCl, and 10% glycerol. Based on the calculation of molecular weight of the human FGF-9 (47 kDa), it is predicted to be a homodimer [Alexander, N.P. et al. (2001) J. Biol. Chem. 276, 4322-4329].
Figure 4. Cell growth
Comparison of growth of NIH-3T3 cells in the absence (A) or presence (B) of human FGF-9
