● Enzynomics Cytokine International unit (IU)
Description
Recombinant human fibroblast growth factor 10 (FGF-10) mature form produced in
E. coli
Synonyms
Keratinocyte growth factor 2, FGF10
Expression Host
Escherichia coli (E. coli)
NCBI Accession Number
NP_004456
Amino Acid Sequence
Leu40-Ser208
N-terminal Sequence Analysis
Leu-Gly-Gln-Asp-Met / Gly-Gln-Asp-Met-Val
Predicted Molecular Mass
19 kDa
Purity
> 98% by SDS-PAGE
Formulation
1.0 mg/ml Human FGF-10 in PBS without carrier proteins
Sterility
Sterilized through a 0.22 µm membrane filter.
Biological Activity
The ED
50 as determined by the dose-dependent proliferation of MCF-7 human breast cancer cell is 5.5 ~ 22.4 ng/ml.
Endotoxin Level
< 0.1 EU/μg of the protein as determined by the Limulus amebocyte lysate (LAL) method.
Shipping
The product is shipped with dry ice. Upon receipt, store it immediately at -80 °C before use.
Storage and Stability
This product can be diluted in sterile 1X PBS to at least 0.1 mg/ml in the presence of 0.1% BSA and stored for a month at 4 °C. Prepare aliquots and freeze at -80 °C for long-term storage. Avoid repeated freeze-thaw cycles and do not vortex.
Figure 1. SDS-PAGE
2 μg of recombinant Human FGF-10 was resolved with SDS-PAGE under reducing condition and visualized by Coomassie Brilliant Blue staining.
Figure 2. Biological Activity
The biological activity of recombinant human FGF-10 was determined by growth of MCF-7 human breast cancer cells.
Figure 3. Cell growth
Comparison of growth of MCF-7 cells in the absence (A) or presence (B) of human FGF-10
