제품 설명
Adenylate monophosphate kinase(AMPK)는
S. cerevisiae 유래 효소로,
E. coli에서 발현시킨 재조합 단백질입니다. 이 효소는 adenylate monophosphate (AMP)를 adenylate diphosphate (ADP)로 전환하는 반응을 촉매합니다.
제품 특징
- Reaction temperature : 30±10℃
제품 구성품
- Adenylate Monophosphate Kinase
Storage buffer
- 10 mM Tris-HCl, (pH 7.4), 300 mM NaCl, 0.1 mM EDTA, 50% Glycerol, 500 μg/ml BSA,1 mM DTT
Figure. AMPK Kinase activity test
Increase in NADH consumption according to the AMPK enzyme concentrations. The reaction mixture (1000 μl) containing adenosine monophosphate and NADH was incubated with increasing amounts of AMPK. The reduced forms of NADH were spectrophotometrically determined at 340 nm.
