제품 설명
PNGase F, Glycerol-free는 글리세롤을 함유하지 않은 PNGase F(제품번호 M050) 제품입니다. PNGase F는 peptide-N-glycosidase F의 줄임말이며, glycosidase/amidase 활성은 N-acetylglucosamine(GlcNAc)과 아스파라긴 잔기 사이의 결합을 절단하여 당단백질에 연결된 올리고당을 분리합니다.
제품 특징
- Molecular weight: 36 kDa
- Reaction temperature: 37℃
- Heat inactivation: 75℃, 10 min
제품 응용
- Removal of carbohydrate residues from proteins
제품 구성품
- PNGase F (Glycerol-free)
- 10X Glycoprotein Denaturing Buffer
- 10X PNGase F Buffer
- 10X Detergent
제품 품질 관리
- Purity: >99% on SDS-PAGE
- Protease-free
Unit의 정의
One unit of the PNGase F (Glycerol-free) enzyme catalyzes the release of >95% N-linked glycan from 10 ug of denatured RNase B in 10 ul at pH 7.5 in 1 hour at 37°C.
Storage buffer
20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH 7.5
주의 사항
- PNGase F is incapable of cleaving N-linked glycans containing core α1-3 Fucose.
- This enzyme should be stored at 4°C separately from the other reaction components stored at -20°C and then its optimal activity and stability last for up to 12 months.
- Repetitive freezing and thawing should be avoided.
Figure 1. Deglycosylation of RNase B by PNGase F enzyme.
Approximately 1 ㎍ of RNase B are denatured with 1X Glycoprotein Denaturing Buffer at 100°C for 10 minutes. After the addition of NP-40 and PNGase F Buffer, each units of PNGase F (Glycerol-free) are added and the reaction mix is incubated for 1 hour at 37°C. Separation of reaction products are visualized by SDS-PAGE.
