제품 설명
PNGase F는 Elizabethkingia miricola 유래의 glycosidase로서, E. coli에서 과발현한 재조합 효소입니다. PNGase F는 peptide-N-glycosidase F의 줄임말이며, glycosidase/amidase 활성은 N-acetylglucosamine(GlcNAc)과 아스파라긴 잔기 사이의 결합을 절단하여 당단백질에 연결된 올리고당을 분리합니다.
제품 특징
- Molecular weight: 36 kDa
- Reaction temperature: 37℃
- Heat inactivation: 75℃ for 10 min
제품 응용
- Removal of carbohydrate residues from proteins
제품 구성품
- PNGase F
- 10X Glycoprotein Denaturing Buffer
- 10X PNGase F Buffer
- 10X Detergent
품질 관리
- Purity: >99% on SDS-PAGE
- Protease-free
Unit 정의
One unit of the PNGase F enzyme catalyzes the release of >95% N-linked glycan from 10 μg of denatured RNase B in 10 μl at pH 7.5 in 1 hr at 37°C.
Storage Buffer
20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, 50% glycerol, pH 7.5
주의 사항
- N-linked glycans containing core α1-3 Fucose will not be cleaved by PNGase F.
Figure 1. Deglycosylation of RNase B by PNGase F enzyme.
Approximately 1 ㎍ of RNase B are denatured with 1X Glycoprotein Denaturing Buffer at 100°C for 10 minutes. After the addition of NP-40 and PNGase F Buffer, each units of PNGase F are added and the reaction mix is incubated for 1 hour at 37°C. Separation of reaction products are visualized by SDS-PAGE.
Figure 2. Deglycosylation of GOD, GDH by PNGase F enzyme.
