제품 설명
Exonuclease I은 exonuclease I 유전자를 E. coli에서 발현시킨 재조합 단백질입니다. 이 효소는 단일가닥 DNA의 3'-hydroxylation된 뉴클레오타이드를 3' 말단부터 3'->5' 방향으로 연속적으로 제거하여 DNA를 분해합니다. 이 효소의 활성은 3'-말단 phosphate에 의해 억제됩니다.
제품 특징
- Molecular weight: 53.4 KDa
- Reaction temperature: 37°C
- Heat inactivation: 80°C for 20 min
제품 응용
- Removal of residual single-stranded DNA, including oligos, from reaction mixes.
제품 구성품
- Exonuclease I (E. coli)
- 10X Exonuclease I (E. coli) Buffer
- Sterile water
품질 관리
- Purity: >99% on SDS-PAGE
- Endonuclease-free
Unit 정의
One unit is defined as the amount of enzyme that will catalyze the release of 10 nmol of acid-soluble nucleotide in 30 minutes at 37°C under standard reaction conditions.
Storage Buffer
10 mM Tris-HCl (pH 7.5), 100 mM NaCl , 0.5 mM EDTA, 5 mM 2-mercaptoethanol, 100 μg/ml BSA, 50% glycerol.
10X Exonuclease I Buffer
670 mM Glycine-KOH (pH 9.5), 67 mM MgCl2, 100 mM 2-mercaptoethanol
Figure 1. Biochemical activity of Exonuclease I (E. coli)
15 fmol of 5’ end-labeled ss/dsDNA substrates were incubated with increasing amount of Exonuclease I (E. coli) at 37℃. Reaction products were analyzed on PAGE.
