제품 설명
T4 Polynucleotide Kinase, 3' Phosphatase 는 3'-phosphatase 활성이 없는 T4 Polynucleotide Kinase(Cat. M005)의 변이체입니다. 이 효소는 T4 Polynucleotide Kinase의 5'-인산화 및 5'-phosphate 교환 반응 활성을 가지고 있습니다.
제품 특징
- Molecular weight: 33 kDa
- Reaction temperature: 37℃
- Heat inactivation: 65℃ for 20 min
제품 응용
- End-labeling of DNA or RNA
- Phosphorylate the 5'-hydroxyl ends of linkers
- Label 5'-hydroxyl ends of DNA with [γ-32P]-ATP
제품 구성품
- T4 Polynucleotide Kinase (3' phosphatase-)
- 10X T4 Polynucleotide Kinase Buffer
- Sterile water
Unit 정의
One unit is defined as the amount of enzyme catalyzing the incorporation of 1 nmol of acid-insoluble [32P] in 30 minutes at 37℃.
Storage
10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 0.1 μM ATP, pH 7.4 @ 25℃, Store at -20℃
10X T4 Polynucleotide Kinase Buffer
700 mM Tris-HCl (pH7.6), 100 mM MgCl2, 50 mM DTT
