제품 설명
Sulfolobus DNA polymerase IV는 proofreading exonuclease 약한 활성을 가지고있으며, 주된 기능으로는 translesion synthesis (TLS)로 손산된 DNA의 복제에 사용됩니다.
제품 활성
- 5’→3’ Exonuclease: No
- 3’→5’ Exonuclease: No
- Strand displacement: No
제품 특징
- Isolated from a recombinant source
- Reaction temperature : 55℃
- Heat inactivation : No
제품 응용
- Translesion synthesis (TLS)
- DNA repair
제품 구성품
- Sulfolobus DNA polymerase Ⅳ
- 10X Sulfolobus DNA pol Ⅳ buffer
제품 품질관리
- Purity : >95% on SDS-PAGE
- Extreme pure(EP)
Unit의 정의
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid insoluble material in 30 minutes at 55°C.
Storage buffer 조성
10 mM Tris-HCl, (pH 7.4), 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol
제품 보관
-20℃
Figure. Sulfolobus DNA polymerase IV activity.
Enzyme activity was measured by incorporation of the radiolabeled nucleotide [
3H]-dTTP into activated calf thymus DNA at 55 °C for 60 minutes.
