제품 설명
EZ Thermo Sequenase는 nTaq (Cat. P025 또는 P050)에 dideoxynucleotides를 DNA에 결합시키는 기능이 추가되어 DNA sequencing에 적합합니다.
Source
An E. coli strain that carries the modified Taq DNA polymerase gene, a genetically engineered form of the native DNA polymerase from Thermus aquaticus.
제품 특징
- Molecular weight: 65 KDa
제품 응용
- DNA Sequencing
제품 구성품
- EZ Thermo Sequenase
- 10X EZ Thermo Sequenase Buffer
- EZ Thermo Sequenase Storage Buffer
- Sterile water
제품 품질관리
- Purity: >99% on SDS-PAGE
- Endonuclease free
- Exonuclease free
Unit의 정의
One Units is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble materials in 30 min at 74℃ in 50 μl reaction mixture.
Storage buffer
- 20mM Tris-HCl (pH 8.5), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% NP-40, 0.5% Tween 20, 50% glycerol.
10X EZ™-Thermo Sequenase Buffer
- 260 mM Tris-HCl (pH 9.5), 65 mM MgCl2
