제품 설명DNA Polymerase I (Klenow)는 E. coli DNA Polymerase I(Cat. DP002)의 일부분으로 Klenow fragment라고도 불립니다. 이 fragment 는 중합 활성과 3’→5’ exonuclease proofreading 활성 및 strand displacement 기능을 가지나 5'→3' exonuclease 활성은 없습니다.
제품 활성
- 5’→3’ Exonuclease: No
- 3’→5’ Exonuclease: Yes
- Strand displacement: Yes
제품 특징
- Molecular weight: 68 kDa
- Reaction temperature: 25℃
- Heat inactivation: 75℃, 20 min
제품 구성품
- Klenow DNA Polymerase (DNA polymerase l large fragment)
- 10X Klenow DNA Polymerase Buffer
- Sterile water
제품 응용
- Cleavage of 3' overhangs to make blunt ends
- Gap filling in 5' overhangs to make blunt ends
- DNA labeling by random priming
- DNA labeling by gap filling in 5' overhangs
- Second strand extension in cDNA synthesis
제품 품질관리
- Purity: >99% on SDS-PAGE
- Endonuclease-free
- Exonuclease-free
- RNase-free
Unit의 정의
One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble materials with 70 mg/ml of denatured herring sperm DNA as template in 30 min at 37℃.
Storage buffer
100 mM KPO4 (pH 6.5), 1 mM DTT, 50% glycerol
10X Klenow DNA Polymerase buffer
100 mM Tris-HCl (pH 7.9), 100 mM MgCl2, 10 mM DTT, 500 mM NaCl
