● Enzynomics Cytokine International unit (IU)
Description
Recombinant human cardiotrophin-like cytokine factor 1 (hCLCF1) mature form produced in E. coli
Synonyms
B-cell-stimulating factor 3, BSF-3, BSF3, CISS2, CLC, NNT-1, NNT1, Novel neurotrophin-1, NR6
Expression Host
Escherichia coli (E. coli)
NCBI Accession Number
Q9UBD9
Amino Acid Sequence
Leu28-Phe225
N-terminal Sequence Analysis
Leu-Asn-Arg-Thr-Gly
Predicted Molecular Mass
22.3 kDa
Purity
> 95% by SDS-PAGE
Formulation
1 mg/ml human CLCF1 in PBS containing 0.25~0.5% Triton X-100 without carrier proteins
Sterility
Sterilized through a 0.22 µm membrane filter.
Biological Activity
The ED
50 as determined by the dose dependent proliferation of SH‑SY5Y human neuroblastoma cell is 0.71~1.13 ng/ml.
Endotoxin Level
< 0.1 EU/μg of the protein as determined by the Limulus amebocyte lysate (LAL) method.
Shipping
The product is shipped with dry ice. Upon receipt, store it immediately at -80 °C before use.
Storage and Stability
This product can be diluted in sterile 1X PBS to at least 0.1 mg/ml and stored for a month at 4 °C. Prepare aliquots and freeze at 80 °C in the presence of 0.1% BSA for long term storage. Avoid repeated freeze thaw cycles.
Citation
1. Kang. J. S., Kim. J. H., Kim. M. J., Min. B., Lee. S., Go. G., Kim. J., Kim. S., Kwak. J. Y., Chun. S., Song. W., Moon. H. Y., Chung. S. G., Park. D., Kim. C., Lee. P., Kwon. E., Kim. N., Kwon. K., & Yang. Y. R. (2025) Exercise-induced CLCF1 attenuates age-related muscle and bone decline in mice. Nature Communications 16:4743
Figure 1. SDS-PAGE
1 μg of recombinant human CLCF1 was resolved with SDS-PAGE under reducing condition and visualized by Coomassie Brilliant Blue staining.
Figure 2. Size analysis
The recombinant human CLCF1 was sedimented through a glycerol gradient and each fraction was analyzed by SDS-PAGE. By comparison with standard proteins, CLCF1 is predicted to be a monomer.
Figure 3. Bioactivity assay
The biological activity of recombinant human CLCF1 was determined by growth of SH‑SY5Y human neuroblastoma cell.
Figure 4. Cell growth
Comparison of growth of SH-SY5Y cells in the absence (A) or presence (B) of human CLCF1
