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FAQ

Home > TECHNICAL > FAQ
How much vector and insert should I use with EZ-Fusion Cloning kit?

A: Use accurate amounts of vector and insert. It is better to use 100 ng of vector if the size is less than 10 kb and 200 ng of the vector if the size is larger than 10 kb.


Calculate the amount of insert DNA as following method.

(Insert size) / (vector size) x (amount of vector) x 2


High efficiency could be obtained by purification of the vector and insert DNA. Increase the total reaction volume to 20 µl if the concentration of insert and vector DNA is low. However, to obtain high efficiency use only 5 µl of EZ-Fusion cloning reaction for transformation.

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