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FAQ

Home > TECHNICAL > FAQ
I got large amount of self colony with TOPcloner™. What is the reason?

A: TOPcloner™ vector contain ccdB gene which reduce the self-ligation of the vector. However binding of topoisomerase to vector may destroy ccdB gene and increase self-ligated colony. Percentage of self-ligated colony may increase if the normal cloning reaction does not work properly.

 


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