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FAQ

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I cannot obtain colony with TOPcloner™. What could be the reason?

A: Reconfirm reaction temperature and time if you failed to obtain colonies with TOPcloner™. Proper reaction temperature for TOPcloner™ is 25 and reaction time is up to 20 minutes. Use the proper amount of insert DNA. The amount of the insert with TOPcloner™ should adjust to 20 ng per 700 bp. Use high efficiency competent cells for transfrmation.

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