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Heat inactivation of restriction endonucleases

Enzyme

Cat.#

Heat Inactivation Temperature

Heat Inactivation Time

Aat II

R026

65°C

20 min

Acc I

R023

80°C

20 min

Afl II

R042

65°C

20 min

Age I

R063

65°C

20 min

EZ-CleanCut Age I

CR063

65°C

20 min

Apa I

R020

65°C

20 min

Asc I

R068

65°C

20 min

Ase I

R069

65°C

20 min

AsiS I

R070

80°C

20 min

Ava I

R034

80°C

20 min

Ava II

R040

80°C

20 min

Bgl I

R043

65°C

20 min

Bsa I

R072

65°C

20 min

BsaW I

R073

80°C

20 min

BsiW I

R074

80°C

20 min

BsmB I

R075

80°C

20 min

BsoB I

R076

80°C

20 min

BstX I

R030

65°C

20 min

BtsC I

R079

80°C

20 min

Cla I

R012

65°C

20 min

Dde I

R044

65°C

20 min

Dpn I

R054

80°C

20 min

Dpn II

R083

65°C

20 min

Dra I

R065

65°C

20 min

EcoO109 I

R085

65°C

20 min

EcoR I

R002

65°C

20 min

EZ-CleanCut EcoR I

CR002

65°C

20 min

EcoR V

R018

65°C

20 min

EZ-CleanCut EcoR V

CR018

65°C

20 min

Fok I

R088

65°C

20 min

Hae II

R090

80°C

20 min

Hae III

R022

80°C

20 min

Hga I

R052

65°C

20 min

Hha I

R045

65°C

20 min

Hinc II

R037

65°C

20 min

Hind III

R008

80°C

20 min

Hinf I

R046

80°C

20 min

HinP1 I

R061

65°C

20 min

Hpa II

R049

80°C

20 min

Hph I

R053

65°C

20 min

Hpy188 I

R092

65°C

20 min

Hpy99 I

R093

65°C

20 min

HpyCH4 V

R094

65°C

20 min

Mbo I

R050

65°C

20 min

Mbo II

R060

65°C

20 min

Mlu I

R032

65°C

20 min

Mnl I

R055

65°C

20 min

Mse I

R062

65°C

20 min

MspA1 I

R058

65°C

20 min

Nar I

R024

65°C

20 min

Nco I

R004

65°C

20 min

EZ-CleanCut Nco I

CR004

80°C

20 min

Nde I

R006

65°C

20 min

Nhe I

R016

65°C

20 min

EZ-CleanCut Nhe I

CR016

80°C

20 min

Nla IV

R096

65°C

20 min

Not I

R001

65°C

20 min

EZ-CleanCut Not I

CR001

65°C

20 min

Nt.BstNB I

R097

80°C

20 min

Ple I

R098

65°C

20 min

Pst I

R019

80°C

20 min

Pvu I

R100

No

20 min

Sac I

R005

65°C

20 min

EZ-CleanCut Sac I

CR005

65°C

20 min

Sac II

R036

65°C

20 min

Sal I

R009

65°C

20 min

EZ-CleanCut Sal I

CR009

65°C

20 min

Sau96 I

R101

80°C

20 min

Sca I

R028

80°C

20 min

EZ-CleanCut Sca I

CR028

80°C

20 min

SgrA I

R103

65°C

20 min

Sma I

R015

65°C

20 min

SnaB I

R027

80°C

20 min

Spe I

R011

80°C

20 min

Sph I

R017

65°C

20 min

EZ-CleanCut Sph I

CR017

65°C

20 min

Ssp I

R105

65°C

20 min

Swa I

R039

65°C

20 min

Xba I

R013

65°C

20 min

Xho I

R007

80°C

20 min

Xma I

R064

65°C

20 min


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