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T3 DNA Ligase 
| No | Product Name | Cat.# | Conc | Size | Price(￦) | Add |
|---|---|---|---|---|---|---|
| 1 | T3 DNA Ligase | M057S | 3,000 unit/μl | 150,000 units | ￦ 79,000 |
|
| 2 | T3 DNA Ligase | M057L | 3,000 unit/μl | 750,000 units | ￦ 316,000 |
|
Product
description
T3
DNA Ligase is an ATP-dependent dsDNA Ligase derived from Bacteriophage T3. This
product catalyzes the phosphodiester linkage between the 3’ hydroxyl group and the 5' phosphate
group of the dsDNA. It exhibits efficient ligation performance in both cohesive
end and blunt end.
Applications
-
Cloning
-
Link adapter, linker to dsDNA
-
Circularization of linear DNA
-
Nick-sealing of dsDNA
-
Site-directed mutagenesis
Supplied
with
- 2X T3 DNA Ligase Reaction
Buffer
Storage
buffer
10
mM Tris-HCl (pH 7.4), 50 mM KCl, 1 mM DTT, 50% Glycerol, 0.1 mM EDTA
Reaction
buffer (1X)
66
mM Tris-HCl (pH 7.6),10 mM MgCl2, 1 mM ATP, 1 mM DTT, 7.5%
Polyethylene glycol (PEG 6000)
Heat
inactivation
No.
Standard
reaction conditions
|
T3
DNA Ligase |
1 μl |
|
2X
T3 DNA Ligase Reaction Buffer |
10 μl |
|
*Vector
DNA (4kb) |
0.02 pmol |
|
*Insert
DNA (1kb) |
0.06 pmol |
|
Distilled
water |
up to 20 μl |
*Vector
: Insert = 1 : 3
→ Incubation at 25℃ for 15 min
Figure 1. Ligation of λ DNA Hind III fragments by T3 DNA Ligase.
λ DNA Hind III fragments (250 ng) was ligated by incubating at 25℃ for 15 min with increasing amounts of T3 DNA Ligase.
● Material Safety Data Sheet
