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Poly(U) Polymerase

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1 Poly(U) Polymerase M054S 2 units/μ 60 units ₩ 105,000 추가
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Description

Poly (U) Polymerase is catalyzes the action of attaching AMP from ATP or attaching UMP from UTP to the 

3 'end of RNA.

 

Characteristics

- Source: E. coli strain

- Molecular weight: 46 kDa

- Reaction temperature: 37

- Heat inactivation: No

 

Applications

- Labeling using UTP in RNA

- Poly (U) tailing in RNA

 

Supplied with

- 10X Poly(U) Pol Reaction Buffer

 

1X Reaction buffer

10 mM Tris-HCl (pH7.9), 50 mM NaCl, 10 mM MgCl2, 1 mM DTT

 

Storage buffer

100 mM NaCl, 10 mM Tris-HCl (pH7.5), 1 mM DTT, 0.1 mM EDTA, 50% Glycerol

 

Reaction Conditions

RNA

> 100 pmol

10X Poly(U) Pol Reaction Buffer

2.5 μl

Poly(U) Polymerase

1 μl

RNase Inhibitor (Cat. No. M007S)

1 μl

rUTP

Final 0.5 mM

Sterile water

up to 25 μl

→ Incubate at 37 for 30 min.




Figure 1. Poly(U) tailing of RNA

Reaction mixtures consist of In vitro synthesized RNA and Poly(U) polymerase were incubated at 37℃ for 1.5 hrs in the presence of UTP. Poly(U) tailed products were analyzed on 2% agarose gel.




● Material Safety Data Sheet

- Enzynomics_MSDS_M054_Poly(U) Polymerase_E

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