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E. coli DNA Ligase

No Product Name Cat.# Conc Size Price(₩) Add
1 E. coli DNA Ligase M053S 10 unit/μl 200 units ₩ 70,000 추가
2 E. coli DNA Ligase M053L 10 unit/μl 1,000 units ₩ 280,000 추가
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Description

E. coli DNA Ligase catalyzes the formation of a phophodiester bond between the 5 'phosphate and the 3' hydroxyl of double-stranded DNA (dsDNA) with sticky ends.

 

Characteristics

- Source: E. coli strain

- Molecular weight: 77 kDa

- Reaction temperature: 16

- Heat inactivation: 65 for 20 min

 

Applications

- The sticky end ligation of double-strand DNA

 

Quality control

- Purity: >99% on SDS-PAGE

- Exonuclease-free

 

1X Reaction buffer

30 mM Tris-HCl (pH8.0), 4 mM MgCl2, 26 μM NAD, 1 mM DTT, 50 μg/ml BSA

 

Storage buffer

300 mM NaCl, 10 mM Tris-HCl (pH7.4), 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 200 μg/ml BSA




Figure 1. Ligation of λ DNA Hind III fragments by E.coli DNA Ligase.
λ DNA Hind III fragments (300 ng) was ligated by incubating at 16℃ for 30 min in the presence of increasing amounts of E.coli DNA Ligase.

● Material Safety Data Sheet

- Enzynomics_MSDS_M053_E. coli DNA Ligase_E

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