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JM109 Chemically Competent E. coli new

No Product Name Cat.# Conc Size Price(₩) Add
1 JM109 Chemically Competent E. coli CP910 - 1.1 ml ₩ 60,000 추가
2 JM109 Chemically Competent E. coli CP911 - 2.1 ml ₩ 100,000 추가
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Product description

This product contains highly efficient competent cells prepared from E. coli JM109. They can be transformed by supercoiled plasmid DNA (pUC19) with a high efficiency (1 x 108 cfu/μg). This product is appropriate for plasmid amplification, transformation of ligated DNA and general cloning.

 

Characteristics

- Strain: JM109

- Genotype: F’ traD36 proA+ proB+ lacIq (lacZ)M15 (lac-proAB) supE44 hsdR17 recA1 gyrA96

thi-1 endA1 relA1 e14- λ-

 

Applications

- Amplification and purification of plasmid DNAs

- Construction of genomic DNA and cDNA libraries

- General cloning

 

Quality control

- Transformation efficiency: ~1 x 108 cfu/μg of pUC19

 

Cautions

- It is appropriate to use such antibiotics at their recommendable concentrations: ampicillin (50 μg/ml), 

  kanamycin (25 μg/ml), and tetracycline (12.5 μg/ml)

- Higher antibiotic concentrations will cause decreased transfection efficiency while lower antibiotic

  concentrations will cause the increased number of satellite colonies.

- False-positive colonies can appear if cells are cultured at 37 for 18-24 hr. 

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