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Thermostable FEN1

No Product Name Cat.# Conc Size Price(₩) Add
1 Thermostable FEN1 M025S 32 units/μl 1,600 units ₩ 81,000 추가
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Product description

FEN1(Flap endonuclease 1) is structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. The enzyme cleaves 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis.


Characteristics

- Purified from E. coli containing a recombinant plasmid encoding the human FEN-1 protein.


Applications 

- Cleavage of flap DNA structure


Component

10X Thermostable FEN1 Reaction Buffer, Thermostable FEN1, Sterile water


Unit Definition

One unit is defined as the amount of enzyme required to cleave 10 pmol of 5´ flap containing oligonucleotide substrate in a total reaction volume of 10 µl for 10 min at 65°C.


10X Thermostable FEN1 Reaction Buffer

200 mM Tris-HCl, 100 mM (NH4)2SO4, 100 mM KCl, 20 mM MgSO4, 1% Triton® X-100, pH 8.8 @ 25°C


Standard reaction conditions

1X Thermostable FEN1 Reaction Buffer, Incubate at 65°C



Figure 1. Cleavage efficiency of FEN1 toward double-flap DNA substrate

Reaction mixtures with 15 fmol of flap DNA substrate were incubated for 15 min at 37˚C or 65˚C



● Material Safety Data Sheet

- Enzynomics_MSDS_M025_Thermostable FEN1_E


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