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T4 RNA Ligase 2, truncated K227Q

No Product Name Cat.# Conc Size Price(₩) Add
1 T4 RNA Ligase 2, truncated K227Q M045S 200 units/μl 4,000 units ₩ 77,000 추가
2 T4 RNA Ligase 2, truncated K227Q M045L 200 units/μl 20,000 units ₩ 308,000 추가
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Product description

T4 RNA Ligase 2, truncated K227Q is a point mutant of T4 Rnl2, truncated containing the K227Q mutation. This enzyme ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ OH end of RNA. The enzyme does not use ATP for ligation but requires pre-adenylated linkers. A lysine 227 is a key residue facilitating adenylyl transfer from adenylated ligation donor substrates to the ligase. Thus, this mutation is useful for reducing undesired ligation products.


Characteristics

- The mutant form of T4 RNA Ligase 2, truncated from E. coli 


Applications

- Ligate pre-adenylated 5´ end of DNA or RNA to the 3´end of RNA


Supply with

- 10X T4 RNA Ligase Reaction Buffer

- PEG8000, 

- Sterile water


Standard reaction conditions

1X T4 RNA Ligase Reaction Buffer, Incubate at 25°C



Figure 1. Assaying the formation of ligation product by T4 RNA Ligase 2, truncated K227Q

Ligation mixtures containing 5’pre-adenylated DNA adapter, 30 mer RNA acceptor and ligase were incubated at 25℃ for 2 hr. Reaction products were analyzed on 15% denaturing PAGE and stained with EtBr.


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