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T4 RNA Ligase 1

No Product Name Cat.# Conc Size Price(₩) Add
1 T4 RNA Ligase 1 M042S 10 units/μl 1,000 units ₩ 71,000 추가
2 T4 RNA Ligase 1 M042L 10 units/μl 5,000 units ₩ 284,000 추가
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Product description

T4 RNA ligase 1 catalyzes the ATP-dependent ligation of single-stranded 5′-phosphoryl terminated nucleic acid donor to single-stranded 3′-hydroxyl terminated nucleic acid acceptor through the formation of a 3'→5' phosphodiester bond. Substrates include single-stranded RNA and DNA as well as numerous nucleotide derivatives.


Characteristics

- Single-strand RNA Ligase from an E. coli


Applications

- Ligation of a single-stranded RNA and DNA


Supply with

- 10X T4 RNA Ligase Reaction Buffer

- 10 mM ATP

- PEG8000

- Sterile water


Standard reaction conditions

*This protocol is intended to ligate a DNA or RNA oligo donor to single-stranded RNA acceptor. 1X T4 RNA Ligase Reaction Buffer supplement with 1 mM ATP and 10% PEG8000. Incubate 2 hour at 25°C



Figure 1. Assaying the formation of ligation product by T4 RNA Ligase 1

Ligation mixtures containing 5’pre-adenylated DNA adapter, 30 mer RNA acceptor and ligase were incubated at 25℃ for 2 hr. Reaction products were analyzed on 15% denaturing PAGE and stained with EtBr.


● Material Safety Data Sheet

- Enzynomics_MSDS_M042_T4 RNA Ligase 1_E


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