컨텐츠 바로가기 영역
주메뉴로 바로가기
본문으로 바로가기

Other Products

Home > PRODUCTS > Other Products

Thermostable 5´ AppDNA/RNA Ligase

No Product Name Cat.# Conc Size Price(₩) Add
1 Thermostable 5´ AppDNA/RNA Ligase M039S 20 μM 10 rxns ₩ 77,000 추가
2 Thermostable 5´ AppDNA/RNA Ligase M039L 20 μM 50 rxns ₩ 308,000 추가
Product Name Cat.# Size Price(₩) EA Total(₩) 삭제
Total : 장바구니에 담기 바로 구매 하기
plus minus 삭제

Product description

Thermostable 5’ AppDNA/RNA Ligase is ATP independent RNA ligase from Methanobacterium thermoautotrophicum (Mth RNA ligase). This enzyme active in ligation of a 5’ pre-adenylated linker to the 3’-OH end of either RNA or ssDNA.


Characteristics

Point mutant of Mth RNA ligase from an E. coli


Applications

Ligate a 5´ pre-adenylated 3´-blocked DNA or RNA sequence tag to the 3´-OH of RNA and ssDNA



Figure 1.  Assaying the formation of ligation product by Thermostable 5’AppDNA/RNA Ligase 

Ligation mixtures containing 5’pre-adenylated DNA adapters, 30 mer RNA acceptor and ligase were incubated at 65℃ for 1 hr. Reaction products were analyzed on 15% denaturing PAGE and stained with EtBr.

List