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RNase H

No Product Name Cat.# Conc Size Price(₩) Add
1 RNase H M036S 5 units/μl 250 units ₩ 73,000 추가
2 RNase H M036L 5 units/μl 1,250 units ₩ 292,000 추가
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Product description

E.coli RNase H (Ribonuclease H ) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA which is hybridized to DNA. This enzyme does not digest single or double-stranded DNA.


Characteristics

- RNase H is purified from E. coli expressing the E. coli RNase H gene on a plasmid.

- Reaction temperature: 37

- Heat inactivation: 65 for 20 min


Applications

- Removal of the RNA strand prior to second-strand cDNA synthesis

- Microarrays


Quality control

- Purity: >99% on SDS-PAGE

- Endonuclease-free

- Exonuclease-free

- RNase-free


Unit definition

One unit is defined as the amount of enzyme that will hydrolyze 1 nmol of the RNA in [3H]-labeled poly(rA).poly(dT), to acid-soluble ribonucleotides in a total reaction volume of 50 μl in 20 minutes at 37℃.


Component

- RNase H

- 10X RNase H Buffer

- Sterile water


Storage buffer

20 mM Tris-HCl (pH 7.5), 100 mM KCl, 10 mM MgCl2, 0.1 mM EDTA, 0.1 mM DTT, 50% glycerol.


Reaction buffer (10X)

500 mM Tris-HCl (pH8.3), 750 mM KCl, 30 mM MgCl2, 100 mM DTT




Figure 1. 5′-end labeled RNA/DNA hybrid substrates were incubated with various amount of RHase H at 37˚C for 15 min. Reaction products were analyzed on 10% PAGE.

● Material Safety Data Sheet

- Enzynomics_MSDS_M036_RNase H_E

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