Product description

RNase Inhibitor, Murine is expressed and purified from E. coli as a recombinant. This product strongly inhibits major nonspecific ribonucleases that include RNase A, B and C. However, it does not inhibit sequence-specific ribonuclease or ribonuclease activities associated with DNA or RNA polymerases; these include RNase T1, S1 nuclease and RNase from Aspergillus species. Mouse RNase Inhibitor has significantly improved resistance to oxidation compared to the human RNase inhibitors, and is stable at low DTT concentrations.

Characteristics

- Isolated from a recombinant source

- Improved resistance to oxidation, compared to human RNase inhibitor

Applications

- In vitro transcription 

- cDNA synthesis

- RT qPCR

- In vitro translation

- Separation and identification of specific ribonuclease activities

- Studies of tumor suppression

Quality control

- Purity : >99% on SDS-PAGE

- Endonuclease-free

- Exonuclease-free

- RNase-free

Unit definition

One unit is defined as the amount of mouse RNase Inhibitor required to inhibit the activity of 5 ng of 

RNase A by 50%

Component

mouse RNase Inhibitor

Sterile water

Storage buffer

20 mM HEPES-KOH(pH 7.6), 50 mM KCl, 8 mM DTT, 50% glycerol

Cautions

For efficient protection of RNA in the reaction, add l units fo mouse RNase Inhibitor to 1 μl of reaction mixture.

Figure 1. Reaction mixtures (20 μl) containing 5 µg of RNA were incubated with RNase A in the absence or presence of various amounts of RNase Inhibitor.

●   Enzynomics_M035_ RNase Inhibitor, Murine_manual

● Material Safety Data Sheet

- Enzynomics_MSDS_M035_RNase Inhibitor, Murine_E