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Endonuclease VIII

No Product Name Cat.# Conc Size Price(₩) Add
1 Endonuclease VIII M034S 10 unit/μl 1,000 units ₩ 81,000 추가
2 Endonuclease VIII M034L 10 unit/μl 5,000 units ₩ 324,000 추가
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Product description

Endonuclease VIII from E. coli acts as both an N-glycosylase and an AP-lyase. The N-glycosylase activity releases damaged pyrimidines from double-stranded DNA, generating an apurinic (AP site). The AP-lyase activity cleaves 3’ and 5’ to the AP site leaving a 5’ phosphate and a 3’ phosphate. Damaged bases recognized and removed by Endonuclease VIII include urea, 5, 6- dihydroxythymine, thymine glycol, 5-hydroxy-5- methylhydanton, uracil glycol, 6-hydroxy-5, 6-dihydrothymine and methyltartronylurea. While Endonuclease VIII is similar to Endonuclease III, Endonuclease VIII has β and δ lyase activity while Endonuclease III has β lyase activity.

 

Characteristics

- Isolated from a recombinant source

- Reaction temperature: 37

- Heat inactivation: 75 for 10 min

 

Applications

- Studies of DNA damage and repair

- Single cell electrophoresis (comet assay)

- DNA structure research

- Alkaline unwinding

 

Quality control

- Purity: >99% on SDS-PAGE

- Endonuclease-free

- Exonuclease-free

 

Unit definition

One unit is defined as the amount of enzyme required to cleave 1 pmol of a 34 mer oligonucleotide duplex containing a single AP site* in a total reaction volume of 10 µl in 1 hour at 37 in 1X Endonuclease VIII Reaction Buffer containing 10 pmol of fluorescently labeled oligonucleotide duplex.

(* An AP site is created by treating 10 pmol of a 34 mer oligonucleotide duplex containing a single uracil residue with 1 unit of Uracil-DNA Glycosylase (UDG) for 2 min at 37.)

 

Storage buffer

10 mM Tris-HCl (pH 8.0), 250 mM NaCl, 0.1 mM EDTA, 50% Glycerol

 

Reaction buffer (10X)

100 mM Tris-HCl (pH 8.0), 750 mM NaCl, 10 mM EDTA


Standard reaction conditions

1X Endonuclease VIII Reaction Buffer, Incubate at 37



 

Figure 1. Cleavage of DNA containing an AP site by Endonuclease VIII

20 pmol of 5’ end-labeled DNA substrates were incubated with Endonuclease VIII for 1 hr at 37℃. Reaction products were analyzed on 15% denaturing PAGE.

● Material Safety Data Sheet

- Enzynomics_MSDS_M034_Endonuclease VIII_E


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