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Pyruvate Kinase

No Product Name Cat.# Conc Size Price(₩) Add
1 Pyruvate Kinase M032S 1.9 unit/μl 1,000 units ₩ 43,000 추가
2 Pyruvate Kinase M032L 1.9 unit/μl 5,000 units ₩ 172,000 추가
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Product description

Enzynomics’ Pyruvate kinase, originated from rabbit muscle is expressed and purified from E. coli and is provided as a buffered aqueous glycerol solution. Pyruvate kinase catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP. This transfer yields one molecule of pyruvate and one molecule of ATP.

 

Unit definition

One unit of enzyme converts 1.0 μmole of phospho(enol)pyruvate to pyruvate per min at pH 7.6

 

Characteristics

- Isolated from a recombinant source

- Molecular weight: 71.5 kDa.

 

Applications

- In vitro enzyme-based ATP regeneration

 

Quality control

- Purity: >99% on SDS-PAGE

- Endonuclease-free

- Exonuclease-free

- RNase-free

 

Storage buffer

1X PBS, 50% glycerol



Figure 1. Reaction mixtures (100 μl) containing phosphoenolpyruvate and NADH were incubated with 20 unit of pyruvate kinase. Reduced forms of NADH was spectrophotometrically determined at 340 nm.

● Material Safety Data Sheet

- Enzynomics_MSDS_M032_RNase Inhibitor_E

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