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RecA
| No | Product Name | Cat.# | Conc | Size | Price(￦) | Add |
|---|---|---|---|---|---|---|
| 1 | RecA | M030S | 2 mg/ml | 200 μg | ￦ 76,000 |
|
| 2 | RecA | M030L | 2 mg/ml | 1,000 μg | ￦ 304,000 |
|
Product description
The RecA protein of Escherichia coli is a multifunctional enzyme that plays a role in homologous recombination, DNA repair and induction of the SOS response. In homologous recombination, the protein functions as a DNA-dependent ATPase, promoting synapsis, heteroduplex formation and strand exchange between homologous DNAs. RecA also acts as a protease cofactor that promotes autodigestion of the lexA product and phage repressors. The proteolytic inactivation of the lexA repressor by an activated form of recA may cause a derepression of the 20 or so genes involved in the SOS response, which regulates DNA repair, induced mutagenesis, delayed cell division and prophage induction in response to DNA damage.
Characteristics
- Isolated from a recombinant source
- Molecular weight: 38 KDa
- Reaction temperature: 37℃
- Heat Inactivation: 65°C for 20 min
Applications
- Visualization of DNA structures with electron microscopy.
- Provide a model system for investigating biochemical steps central to genetic recombination.
- D-loop mutagenesis.
Quality control
- Purity: >99% on SDS-PAGE
- Endonuclease-free
- Exonuclease-free
- RNase-free
Unit definition
Sold by mass of pure protein as determined by OD280.
Storage buffer
10 mM Tris-HCl (pH 7.4), 1 mM DTT, 0.1 mM EDTA, 50% Glycerol
Reaction buffer (10X)
700 mM Tris-HCl (pH 7.6), 100 mM MgCl2, 50 mM dithiothreitol
Cautions
ATPγS which is required for triple helix formation is not supplied.
Standard reaction conditions
1X RecA (E. coli) Buffer, Incubate at 37°C.
Figure 1. DNA bound protein complexes were analyzed on 6% PAGE at 4°C.
