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20X AP-Mix

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1 20X AP-Mix M033S 20X 500 rxns ₩ 64,000 추가
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This product is a 20X mix version of Antarctic Phosphatase (Cat. # M026).

 

Product description

20X AP-Mix (Antarctic Phosphatase 20X PreMIX) removes 5´ phosphate from DNA, RNA, rNTPs and dNTPs. Since phosphatase-treated fragments lack the 5´phosphoryl termini required by ligases, they cannot self-ligate. This property can be used to decrease the vector background in cloning strategies.

20X AP-Mix is ready-to-use solution of Antartic Phosphatase for convinient use. 1 ul of 20X AP-Mix can completely dephosphorylate 1 ug of linearized plasmid DNA in 20 ul reaction volumn within 1 hr at 37.

 

Characteristics

- Remove 5´ phosphate from DNA, RNA, rNTPs and dNTPs.

 

Applications 

- Prevents recircularization of cloning vectors

- Preparation of templates for 5´ end labeling

- Removal of dNTPs and pyrophosphate from PCR reactions

- Dephosphorylation of proteins

 

Cautions

Avoid continuous freez and melt cycles

 

Standard conditions

Linearized plasmid DNA (~2 ug)

~19 μl

20X AP-Mix

1 μl

Sterile water

Up to 20 μl

→Incubate at 37 for 1 hr.

→Inactivate at 70 for 5 min or purify DNA for subsequent application.




Figure 1. 1 μg of pcDNA3.1 cut with Hind III (5´ protruding ends), were incubated with AP mix for 30 min at 37°C. Dephosphorylation is defined as inhibition of recirculation in a self-ligation reaction and is measured by transformation into E. coli.

● Material Safety Data Sheet

Enzynomics_MSDS_M033_20X AP-Mix_E

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