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Antarctic Phosphatase

No Product Name Cat.# Conc Size Price(₩) Add
1 Antarctic Phosphatase M026S 5 units/μl 1,000 units ₩ 70,000 추가
2 Antarctic Phosphatase M026L 5 units/μl 5,000 units ₩ 280,000 추가
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Product description

Antarctic Phosphatase catalyzes the removal of 5´ phosphate from DNA and RNA. Since phosphatase-treated fragments lack the 5´ phosphoryl termini required by ligases, they cannot self-ligate. This property can be used to decrease the vector background in cloning strategies.

 

Characteristics

- Isolated from a recombinant source

- Reaction temperature: 37

- Heat inactivation: 70 for 5 min  

 

Applications

- Removing 5´ phosphates from DNA, RNA, rNTPs and dNTPs.

- Prevention of recircularization of cloning vectors.

- Removal of dNTPs and pyrophosphate from PCR reactions.

 

Quality control                       

- Purity: >99% on SDS-PAGE

- Endonuclease-free

- Exonuclease-free

- RNase-free

 

Unit definition

One unit is defined as the amount of enzyme that will dephosphorylate 1 μg of pUC19 vector DNA cut with Hind III (5´ protruding ends), Hinc II (blunts ends) or Pst I (5´ recessed ends) in 30 minutes at 37°C. Dephosphorylation is defined as > 95% inhibition of recirculation in a self-ligation reaction and is measured by transformation into E. coli.

 

Component

- Antarctic Phosphatase

- 10X Antarctic Phosphatase Buffer

- Sterile water 

 

Storage buffer

10 mM Tris-HCl (pH 7.4), 1 mM MgCl2, 0.01 mM ZnCl2, 1 mM DTT, 50% Glycerol. 

 

Reaction buffer (10X)

500 mM Bis-Tris-Propane HCl, 10 mM MgCl2, 1 mM ZnCl2, pH 6.0  

 

Cautions

Antarctic Phosphatase requires 0.1 mM Zn2+ for activity.



Figure 1. Removal of 5’phosphates from ss/dsDNA by Antartic Phosphatase

15 fmol of 5’ end-labeled ss/dsDNA substrates were incubated with 5 unit of Antarctic Phosphatase at 25. Reaction products were removed at indicated time period and analyzed on PAGE.

● Material Safety Data Sheet

Enzynomics_MSDS_M026_Antarctic Phosphatase_E

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