컨텐츠 바로가기 영역
주메뉴로 바로가기
본문으로 바로가기

Other Products

Home > PRODUCTS > Other Products

Endonuclease IV

No Product Name Cat.# Conc Size Price(₩) Add
1 Endonuclease IV M028S 10 units/μl 1,000 units ₩ 77,000 추가
2 Endonuclease IV M028L 10 units/μl 5,000 units ₩ 308,000 추가
Product Name Cat.# Size Price(₩) EA Total(₩) 삭제
Total : 장바구니에 담기 바로 구매 하기
plus minus 삭제

Product description

Endonuclease IV is a class apurinic/apyrimidinic (AP) endonuclease that will hydrolyse intact AP sites in DNA. AP sites are cleaved at the first phosphodiester bond that is 5´ to the lesion leaving a hydroxyl group at the 3´ terminus and a deoxyribose 5´-phosphate at the 5´ terminus. The enzyme also has a 3´ -diesterease activity and can release phosphoglycoaldehyde, intact deoxyribose 5-phosphate and phosphate from the 3´ end of DNA.

 

Characteristics

- Isolated from a recombinant source

- Reaction temperature: 37

- Heat inactivation: 85 for 20 min

 

Applications

- Studies of DNA damage and repair

- Single cell electrophoresis (comet assay)

- DNA structure research

- Alkaline unwinding

 

Quality control

- Purity: >99% on SDS-PAGE

- Endonuclease-free

- Exonuclease-free

 

Unit definition

One unit is defined as the amount of enzyme required to cleave 1 pmol of a 34-mer oligonucleotide duplex containing a single AP site* in a total reaction volume of 10 µl in 1 hour at 37°C.

(* An AP site is created by treating 10 pmol of a 34 mer oligonucleotide duplex containing a single uracil residue with 1 unit of Uracil-DNA Glycosylase (UDG) for 2 min at 37.)

 

Component

- Endonuclease IV

- 10X Endonuclease IV Buffer

- Sterile water

 

Storage buffer

10 mM Tris-HCl, 250 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.15% Triton X-100, 200 ug/ml BSA, 50% Glycerol,

pH 7.4

 

Reaction buffer (10X)

500 mM Tris-HCl, 1000 mM NaCl, 100 mM MgCl2, 10 mM DTT, pH 7.9

 

Standard reaction conditions

1X Endonuclease IV Buffer, incubate at 37°C.



Figure 1. Cleavage of DNA containing an AP site by Endonuclease IV

20 fmol of 5’ end-labeled dsDNA substrates were incubated with Endonuclease IV at 37˚C. Cleaved DNA were analyzed on 15% Urea polyacrylamide gels in 0.5X TBE.

● Material Safety Data Sheet

Enzynomics_MSDS_M028_Endonuclease IV_E


List