컨텐츠 바로가기 영역
주메뉴로 바로가기
본문으로 바로가기

Other Products

Home > PRODUCTS > Other Products

Proteinase K

No Product Name Cat.# Conc Size Price(₩) Add
1 Proteinase K PR003S 20 mg/ml 1 ml ₩ 20,000 추가
2 Proteinase K PR003M 20 mg/ml 2 ml ₩ 36,000 추가
3 Proteinase K PR003L 20 mg/ml 5 ml ₩ 80,000 추가
Product Name Cat.# Size Price(₩) EA Total(₩) 삭제
Total : 장바구니에 담기 바로 구매 하기
plus minus 삭제

Product description

Proteinase K is a non-specific, subtilisin-related serine protease with a very high specific activity. Proteinase K is not inactivated by chelating reagents such as EDTA or detergents such as SDS and is active over a wide range of pH


Characteristics

- Specific Activity: > 30 units/mg

- Molecular Weight: 28.9 kDa


Applications

- Isolation of plasmid and genomic DNA

- Isolation of RNA

- Inactivation of RNase, DNase and enzymes in reactions


Quality control

- Purity: >99% on SDS-PAGE

- Endonuclease-free

- Exonuclease-free
-
RNase-free


Unit Definition

One unit is defined as the amount of enzyme required to liberate folin-positive amino acids and peptides corresponding to 1 µmol of tyrosine in 1 minute at 37 in a total reaction volume of 250 µl


Storage Conditions

10 mM Tris-HCl (pH 7.5), 1 mM CaCl2, 50% Glycerol Store at -20


Reaction Conditions

Proteinase K is active in a wide range of buffers (pH 4.0~12.5) temperatures (37~60°C) and salts. It is also active in detergents such as Tween 20 (5%), Triton X-100 (1%) and SDS (1%).


Cautions

- Proteinase K is inhibited by diisopropyl fluorophosphate and phenylmethylsulfonyl fluoride (PMSF)

- To stimulate proteinase K activity, add denaturing agents (SDS)

- Proteinase K is 12 times more active at 65℃ then at 25℃. However, it is rapidly denatured at

  temperatures above 65℃

● Material Safety Data Sheet

- Enzynomics_MSDS_PR003_Proteinase K_E

List