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Benzonase

No Product Name Cat.# Conc Size Price(₩) Add
1 Benzonase M018S 25 units/μl 2,500 units ₩ 40,000 추가
2 Benzonase M018L 25 units/μl 10,000 units ₩ 120,000 추가
3 Benzonase M018H 250 units/μl 25,000 units ₩ 180,000 추가
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Product description

Genetically engineered Benzonase Nuclease from Serratia marcescens degrades all forms of DNA and RNA (single-stranded, double-stranded, linear and circular) while having no proteolytic activity. It is effective over a wide range of conditions and possesses an exceptionally high specific activity. It completely digests nucleic acids to 5’-monophosphate terminated oligonucleotides 3 to 5 bases in length (below the hybridization limit), which is ideal for removal of nucleic acids contamination.

 

Characteristics

Benzonase degrades all forms of DNA and RNA while having exceptionally high specific activity. It is active over wide range for buffer conditions and free from detectable proteolytic activity.

            

Applications

- Effective removal of nucleic acids

- Viscosity reduction from protein solutions

- Enhanced protein purification

- Increased gel resolution

- Prevention of cell clumping

 

Quality control

Produced as a single band on SDS-PAGE

            

Unit Definition

One unit of Benzonase Nuclease is defined as the amount of enzyme that results in an ΔA260 of 1.0 in 30 min, which corresponds to complete digestion of 37 μg DNA.

 

Storage

50 mM Tris-HCl pH 8.0, 20 mM NaCl, 2 mM MgCl2,

50% Glycerol, store at –20 DO NOT store at –70

 

Heat Inactivation

65 for 20 min

 

Standard reaction conditions         

Example: Removal of nucleic acids

 Substrate solution: supercoiled plasmid DNA solution in 50 mM Tris-HCl pH 8.0, 1 mM MgCl2, 0.1 mg/ml BSA.

Plasmid DNA (0.05 μg/μl)

20 μl

Benzonase (dilution sample)

1 μl

• Dilution buffer: 50 mM Tris-HCl pH 8.0, 20 mM NaCl, 2 mM MgCl2

→ Incubate the reaction mixture at 37.

→ withdraw 10 μl aliquots at 1, 2, 4, and 6 hr after incubation

→ add 2 μl of 6X sample buffer and mix.

→ store at -20 until required for electrophoresis.

 


Figure 1. 1 μg of pSK-M2 plasmid was digested with indicated units of Benzonase at 37℃ for 10 minutes. The reaction products were analyzed with agarose gel electrophoresis.

● Material Safety Data Sheet

Enzynomics_MSDS_M018_Benzonase_E

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