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Tth RecA

No Product Name Cat.# Conc Size Price(₩) Add
1 Tth RecA M016S 1 mg/ml 100 μg ₩ 160,000 추가
2 Tth RecA M016L 1 mg/ml 500 μg ₩ 640,000 추가
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Product description

A gene encoding Tth RecA was cloned from Thermus thermophiles and expressed in E. coli, and the enzyme was purified to homogeneity. The RecA protein is essential for homologous recombination and for the postreplicative repair of damage to DNA. The extreme thermostability makes Tth RecA ideal for molecular biology applications that require an elevated temperature condition, such as nucleic acid amplification and sequencing.

 

Characteristics

- Molecular weight: 36 kDa

- Optimal temperature: 65~75

- Heat inactivation: No

            

Applications

- Multiplex PCR

- Site-directed mutagenesis through D-loop

 

Quality control

- Purity: >99% on SDS-PAGE

- Endonuclease-free

- Exonuclease-free

- phosphatase-free

            

Unit Definition

Sold by mass of pure protein as determined by OD280.

 

Storage

10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 0.1% Triton® X-100 pH 7.5 @ 25, Store at -20.

 

Standard reaction conditions         

- PCR mixture

10X nTaq buffer

10 μl

nTaq DNA Polymerase (5 units/μl)

1 μl

dNTP mixture (2 mM each, final conc., 200 μM each)

10 μl

Template DNA (0.1-500 ng/μl)

1 μl

Primer 1 (5 pmol/μl)

5 μl

Primer 2 (5 pmol/μl)

5 μl

Tth RecA

0.8 μg

Distilled water

Up to 100 μl

Assemble the reaction mixture on ice

 

● Material Safety Data Sheet

Enzynomics_MSDS_M016_Tth RecA_E

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