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Uracil-DNA Glycosylase (Heat-labile)
| No | Product Name | Cat.# | Conc | Size | Price(￦) | Add |
|---|---|---|---|---|---|---|
| 1 | Uracil-DNA Glycosylase (Heat-labile) | M013S | 1 units /μl | 100 units | ￦ 75,000 |
|
| 2 | Uracil-DNA Glycosylase (Heat-labile) | M013L | 1 units /μl | 500 units | ￦ 300,000 |
|
Product description
Uracil-DNA
Glycosylase (Heat-labile) is a recombinant enzyme from marine bacterium BMTU
3346.
UDG
catalyses the hydrolysis of the N-glycosylic bond between the deoxyribose sugar
and the base in uracil-containing DNA (uracil-DNA).
Characteristics
-
Molecular weight: 27.1 kDa
-
Reaction temperature: 15~25℃
-
Heat inactivation: Half life of 2 min at 40℃
Applications
-
Control of carry-over contamination in PCR
-
Cloning of PCR products
-
Used to investigate features of protein-DNA interactions
Quality
control
-
Purity: >99% on SDS-PAGE
-
Endonuclease-free
-
Exonuclease-free
-
Phosphatase-free
Unit
Definition
One
unit is defined as the amount of enzyme that catalyzes the release of 60 pmol
of uracil per minute from double-stranded, uracil containing DNA.
Storage
20
mM Tris-HCl (pH 7.8), 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.05% Tween 20, 50%
Glycerol, Store at -20℃.
10X
Uracil-DNA Glycosylase (Heat-labile) buffer
200 mM Tris-HCl, 10 mM EDTA, 10 mM DTT (pH 8.0 @ 25℃).
Standard
reaction
conditions
Use
of UDG to Control PCR
1. Replace dTTP by 200 - 600 μM dUTP in all PCR reaction
2.
Add 1 unit of UDG per 100 μl
PCR reaction.
3.
Incubate all PCR reactions at 15℃ to 25℃ for 10 min.
4.
Inactivate UDG by incubation at 95℃ for 10 min.
5.
After PCR, the product may be stored at 2-8℃.
Cautions
- Inhibition by high ionic strength (> 100 mM).
● Material Safety Data Sheet
- Enzynomics_MSDS_M013_Uracil-DNA Glycosylase (Heat-labile)_E
