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Uracil-DNA Glycosylase (Heat-labile)

No Product Name Cat.# Conc Size Price(₩) Add
1 Uracil-DNA Glycosylase (Heat-labile) M013S 1 units /μl 100 units ₩ 75,000 추가
2 Uracil-DNA Glycosylase (Heat-labile) M013L 1 units /μl 500 units ₩ 300,000 추가
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Product description

Uracil-DNA Glycosylase (Heat-labile) is a recombinant enzyme from marine bacterium BMTU 3346.

UDG catalyses the hydrolysis of the N-glycosylic bond between the deoxyribose sugar and the base in uracil-containing DNA (uracil-DNA).

 

Characteristics

- Molecular weight: 27.1 kDa

- Reaction temperature: 15~25

- Heat inactivation: Half life of 2 min at 40

            

Applications

- Control of carry-over contamination in PCR 

- Cloning of PCR products 

- Used to investigate features of protein-DNA interactions

 

Quality control

- Purity: >99% on SDS-PAGE

- Endonuclease-free

- Exonuclease-free

- Phosphatase-free

            

Unit Definition

One unit is defined as the amount of enzyme that catalyzes the release of 60 pmol of uracil per minute from double-stranded, uracil containing DNA.

 

Storage

20 mM Tris-HCl (pH 7.8), 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.05% Tween 20, 50% Glycerol, Store at -20.

 

10X Uracil-DNA Glycosylase (Heat-labile) buffer

200 mM Tris-HCl, 10 mM EDTA, 10 mM DTT (pH 8.0 @ 25).

 

Standard reaction conditions             

Use of UDG to Control PCR
1. Replace dTTP by 200 - 600 μM dUTP in all PCR reaction

2. Add 1 unit of UDG per 100 μl PCR reaction.

3. Incubate all PCR reactions at 15 to 25  for 10 min.

4. Inactivate UDG by incubation at 95 for 10 min.

5. After PCR, the product may be stored at 2-8.

 

Cautions

- Inhibition by high ionic strength (> 100 mM).

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