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Uracil-DNA Glycosylase (E.coli )
| No | Product Name | Cat.# | Conc | Size | Price(￦) | Add |
|---|---|---|---|---|---|---|
| 1 | Uracil-DNA Glycosylase (E.coli ) | M012S | 5 units/μl | 1,000 units | ￦ 73,000 |
|
| 2 | Uracil-DNA Glycosylase (E.coli ) | M012L | 5 units/μl | 5,000 units | ￦ 300,000 |
|
Product description
UDG
catalyses the hydrolysis of the N-glycosylic bond between the deoxyribose sugar
and the base in uracil-containing DNA (uracil-DNA). The enzyme shows no
measurable activity on short oligonucleotides (<6 bases), or RNA substrates.
Characteristics
-
Molecular weight: 24.5 kDa
-
Reaction temperature: 37℃
-
Heat inactivation: No
Applications
-
Control of carry-over contamination in PCR
-
Cloning of PCR products
-
Used to investigate features of protein-DNA interactions
Quality
control
-
Purity: >99% on SDS-PAGE
-
Endonuclease-free
-
Exonuclease-free
-
Phosphatase-free
Unit
Definition
One
unit is defined as the amount of enzyme that catalyzes the release of 60 pmol
of uracil per minute from double-stranded, uracilcontaining DNA.
Storage
10
mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.1 mg/ml BSA, 50% Glycerol pH
7.4 @ 25℃, Store at -20℃.
10X
Uracil-DNA Glycosylase (E.coli) buffer
200 mM Tris-HCl, 10 mM EDTA, 10 mM DTT (pH 8.0 @ 25℃).
Standard
reaction
conditions
Use
of UDG to Control PCR
1. Replace dTTP by 200 - 600 μM dUTP in all PCR reaction
2.
Add 1 unit of UDG per 100 μl
PCR reaction.
3.
Incubate all PCR reactions at 37℃ for 10 min.
4.
Inactivate UDG by incubation at 95℃ for 10 min.
5.
After PCR, the product may be stored at 2-8℃.
Cautions
- UDG is active over a broad pH range with an optimum at pH 8.0, does not require a divalent cation
- Inhibition by high ionic strength (> 200 mM)
● Material Safety Data Sheet
