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Uracil-DNA Glycosylase (E.coli )

No Product Name Cat.# Conc Size Price(₩) Add
1 Uracil-DNA Glycosylase (E.coli ) M012S 5 units/μl 1,000 units ₩ 73,000 추가
2 Uracil-DNA Glycosylase (E.coli ) M012L 5 units/μl 5,000 units ₩ 300,000 추가
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Product description

UDG catalyses the hydrolysis of the N-glycosylic bond between the deoxyribose sugar and the base in uracil-containing DNA (uracil-DNA). The enzyme shows no measurable activity on short oligonucleotides (<6 bases), or RNA substrates.

 

Characteristics

- Molecular weight: 24.5 kDa

- Reaction temperature: 37

- Heat inactivation: No

            

Applications

- Control of carry-over contamination in PCR 

- Cloning of PCR products 

- Used to investigate features of protein-DNA interactions

 

Quality control

- Purity: >99% on SDS-PAGE

- Endonuclease-free

- Exonuclease-free

- Phosphatase-free

            

Unit Definition

One unit is defined as the amount of enzyme that catalyzes the release of 60 pmol of uracil per minute from double-stranded, uracilcontaining DNA.

 

Storage

10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.1 mg/ml BSA, 50% Glycerol pH 7.4 @ 25, Store at -20.

 

10X Uracil-DNA Glycosylase (E.coli) buffer

200 mM Tris-HCl, 10 mM EDTA, 10 mM DTT (pH 8.0 @ 25).

 

Standard reaction conditions             

Use of UDG to Control PCR
1. Replace dTTP by 200 - 600 μM dUTP in all PCR reaction 

2. Add 1 unit of UDG per 100 μl PCR reaction.

3. Incubate all PCR reactions at 37 for 10 min. 

4. Inactivate UDG by incubation at 95 for 10 min.   

5. After PCR, the product may be stored at 2-8


Cautions

- UDG is active over a broad pH range with an optimum at pH 8.0, does not require a divalent cation

- Inhibition by high ionic strength (> 200 mM)

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