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Exonuclease I (E. coli )

No Product Name Cat.# Conc Size Price(₩) Add
1 Exonuclease I (E. coli ) M024S 20 units /μl 3,000 units ₩ 70,000 추가
2 Exonuclease I (E. coli ) M024L 20 units /μl 15,000 units ₩ 280,000 추가
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Description

Exonuclease I (E. coli) is a monomeric enzyme that processively degrades single stranded DNA in the 3' to 5' direction. The enzyme is processed through digestion and is inhibited by the presence of a 3'-terminal phosphate.

 

Characteristics

-Molecular weight: 53.4 KDa

-Reaction temperature: 37°C

-Heat inactivation: 80°C for 20 min

 

Applications

-Removal of residual ssDNA, including oligos, from reaction mixes

 

Quality Control

-Purity: >99% on SDS-PAGE

-Endonuclease-free

 

Unit Definition

One unit is defined as the amount of enzyme that will catalyze the release of 10 nmol of acid-soluble nucleotide in 30 minutes at 37°C under standard reaction conditions..

 

Storage Conditions

10 mM Tris-HCl (pH 7.5), 100 mM NaCl , 0.5 mM EDTA, 5 mM 2-mercaptoethanol, 100 μg/ml BSA, 50% glycerol.

 

10X Exonuclease I (E. coli) buffer

670 mM Glycine-KOH (pH 9.5), 67 mM MgCl2, 100 mM 2-mercaptoethanol

 

Standard reaction conditions

ssDNA (100 mers)

2.5 μg

10X Exonuclease I Buffer

2 μl

Exonuclease I

10 ~ 50 units

Distilled water

Up to 20 μl

→ Incubate the reaction at 37 for 30 min.



● Material Safety Data Sheet

- Enzynomics_MSDS_M024_Exonuclease I (E. coli)_E

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