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TNFα

No Product Name Cat.# Conc Size Price(₩) Add
1 TNFα C001S 1 mg/ml 50 μg ₩ 150,000 추가
2 TNFα C001M 1 mg/ml 0.5 mg ₩ 900,000 추가
3 TNFα C001L 1 mg/ml 1 mg ₩ 1,500,000 추가
Product Name Cat.# Size Price(₩) EA Total(₩) 삭제
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Product Description
TNFα is a carrier-free recombinant protein that is expressed and purified from E. coli. It is purified to near homogeneity by the use of several chromatographic steps. It is a wild-type protein ith 157 amino acids with a methionine residue at its Nterminus. Its biological activity has been confirmed with a cytotoxic assay carried out with murine L929 cells. Its activity is specifically inactivated by neutralizing anti-TNFα antibodies.

Characteristics
- Molecular weight : 17.5 kDa
- Full length, recombinant
- Concentration : 1 mg/ml (This product can be diluted in 1X PBS to obtain 100-500 μg/ml final concentration)

Applications
- ELISA
- Western Blotting

Quality control
- Purity : >97% on SDS-PAGE

Biological activity
ED50 range = 0.02-0.05 ng/ml
(In a cytotoxic assay measured in a murine L929 cell line treated together with actinomycin D)

Storage conditions
- This product is provided in 1X PBS, pH 7.4 (137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 2 mM KH2PO4)
- Store at -20 or below.
- Avoid repeated cycles of freezing-thawing
  It is recommended to make small aliquots (each for a single use) prior to storage


Figure 1. Purification of recombinant TNFα to homogeneity.

In order to confirm the purity of recombinant TNFα (Cat.# C001), increasing amounts (0.25 to 6 μg) of recombinant TNFα was subjected to analysis in 15% SDS-PAGE. Impurities were not detectable with the highest levels (6 μg) of purified TNFα. Mw, molecular weight marker.

 

● Material Safety Data Sheet

- Enzynomics_MSDS_C001S_TNFα_E

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