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EZ-Seq™ Nano DNA Library Prep Kit (Index A) new

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1 EZ-Seq™ Nano DNA Library Prep Kit (Index A) EZ034A - 24 rxns ₩ 900,000 추가
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Product description

Enzynomics EZ-Seq™ Nano DNA Library Prep Kit 차세대 염기서열분석을 위한 DNA 샘플의 library 제작에 필요한 모든 구성을 갖춘 제품으로 350 bp 550 bp 사이즈의 insert DNA 사용하는 whole-genome resequencing 최적화되었습니다. 제품은 index 종류에 따라 A, B kit 구성되어 각각 24 샘플에 사용 가능하며 master-mix 형태의 효소를 제공함으로써 제품 구성을 간소화하고 효율성을 높였습니다. Library 제작을 위해 DNA fragment 양쪽 말단에 index adaptor 연결하여 증폭하는 과정에서 단계별 fragment 사이즈 선별과 정제를 위한 bead 제품에 포함되어 있습니다. 제작된 library single-read 또는 paired-end 염기서열분석을 수행할 있습니다.

 

Characteristics

- NGS library preparation kit (illumina sequencing system )

 

Applications

- Human genome, mammalian genome, large or complex genome, microbial genome NGS 분석용 library 제작 키트

 

Components

Box 구성

구성품

용량

a)Box 1

End Repair Mix

1 ml

dA-Tailing Mix

0.7 ml

Adaptor Ligation Mix

0.14 ml

Stop Ligation Buffer

0.5 ml

PCR Primer Cocktail

0.35 ml

Enhanced PCR Mix

0.55 ml

DNA adaptor Indexes (12 Indexes)

each 0.05 ml

b)Box 2

Resuspension Buffer

20 ml

EZ-Pure™ SPRI Bead Mix

10 ml

a)Box 1. -20 보관

b)Box 2. 4 보관

 



그림1. Library 제작 과정 모식도

● Material Safety Data Sheet

Enzynomics_MSDS_EZ034A_EZ-Seq™ Nano DNA Library Prep Kit (Index A)_E


● Quality Data

Figure 1. End repair of DNA ends with 3’, 5’ overhangs

Oligoduplex with 3’, 5’ overhangs and non-phosphorylated DNA were repaired using End Repair Mix. Reaction products were analyzed on 10% native PAGE.



Figure 2. dA-tailing of repaired blunt end DNA

Reaction mixtures with 100 pmol of repaired blunt end DNA substrates were incubated with dA-Tailing Mix at 37℃. For the assessment of dA-Tailing reaction efficiency, self ligation reaction was performed with 20 pmol of dA-tailed DNA. Reaction products were analyzed on 10% native PAGE.



Figure 3. The result of adaptor ligation reaction

30-nt dT-tailed dsDNA adaptors were covalently attached to 20-nt dA-tailed dsDNA using Adaptor Ligation Mix. Reaction mixtures were incubated at 25℃, followed by analyzed on 10% denaturing PAGE.



Figure 4. Library product size distribution

Indexed libraries were constructed from 100 ng human gDNA using EZ-Seq™ Nano DNA Library Prep Kit or Compony I Library Prep Kit. To verify the size of PCR enriched fragments, the template size distribution of individual libraries were measured by running on a Agilent Bioanalyzer.



Figure 5. Alignment Result of yeast gDNA

Indexed libraries were constructed from yeast gDNA using EZ-Seq™ Nano DNA Library Prep Kit or Company I Library Prep Kit. Then, Libraries were sequenced on an Illumina Hiseq 2500 and aligned.



Figure 6. Alignment Result of Human gDNA

Indexed libraries were constructed from human gDNA using EZ-Seq™ Nano DNA Library Prep Kit or Company I Library Prep Kit. Then, Libraries were sequenced on an Illumina Hiseq 2500 and aligned.



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