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EZ-Pure™ Plasmid Prep Kit Ver.2

No Product Name Cat.# Conc Size Price(₩) Add
1 EZ-Pure™ Plasmid Prep Kit Ver.2 EP101-50N - 50 preps ₩ 60,000 추가
2 EZ-Pure™ Plasmid Prep Kit Ver.2 EP101-200N - 200 preps ₩ 130,000 추가
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Introduction

EZ-Pure™ Plasmid Prep provides a rapid, simple and efficient method for plasmid minipreparation and routine molecular biology applications. The kit procedure is relied on alkaline lysis method followed by the process of binding plasmid DNA on a glass filter membrane. After alkaline treatment and centrifugation, the plasmid DNA is remained in the supernatant, whereas the bacterial chromosome, proteins, and other cell debris have been precipitated. The supernatant is subsequently passed through the filter membrane where plasmid DNA is bound selectively. The bound plasmid DNA is then washed and eluted by adding low salt buffers. The resulting isolated plasmid DNA can be used immediately in a variety of additional down-stream applications.


Product Qualification

EZ-Pure™ Plasmid Prep Kit ver.2 is manufactured in a strictly clean condition, and its degree of cleanness is monitored periodically. For quality control, performance of restriction enzyme digestion, PCR amplification, and spectrophotometric analysis are enough.


Storage

EZ-Pure™ plasmid prep Kit ver.2 component should be stored at room temperature (15–25).

RNase A should be stored at 4 for maximum performance. All components are guaranteed to be stable for 

1 year.

● Material Safety Data Sheet

- Enzynomics_MSDS_EP101_EZ-Pure™ Plasmid Prep Kit_Kor


● Quality Data

                                             (A)                                                                             (B)

 Fig 1. Compared with other company plasmid prep kits

(A) pcDNA3.1 plasmid DNA was purified from 5 ml overnight cultured DH5α using EZ-Pure Plasmid prep Kit ver.2 and other company kit. Plasmid DNA was eluted with the indicated volume and resolved on 1% agarose gel. And then plasmid DNA was analyzed in DNA yield and purity. 

(B) Excellent recovery of plasmid DNA pcDNA3.1 from increasing culture volumes of DH5α grown in LA medium using EZ-Pure™ Plasmid prep Kit ver.2 and run on 1% TAE agarose gel. Enzynomics, supplier A~C (lane 2~4 respectively), 100 bp DNA Ladder Marker (M)

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