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TOPcloner™ TA-Blunt core Kit

No Product Name Cat.# Conc Size Price(₩) Add
1 TOPcloner™ TA-Blunt core Kit EZ017S - 20 rxns ₩ 200,000 추가
2 TOPcloner™ TA-Blunt core Kit EZ017M - 40 rxns ₩ 360,000 추가
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Product description

TOPcloner™ TA-Blunt core kit are suitable for cloning at room temperature in 5 min with both Taq polymerase-amplified PCR products (A tail overhangs) and Pfu polymerase-amplified PCR products (blunt ends). The vector provided in this kit contains topoisomerase covalently coupled to its ends, which allows efficient cloning of any DNA fragments to the vector without using a regular ligase.

 

Characteristics

- Short reaction time (5 min)

- Highly efficient cloning

- Direct use of PCR products

 

Applications

- Fast and efficient cloning of both A-tailed and blunt ended PCR products

 

Supplied with

- pTOP TA-Blunt V2

- 6X TOPcloner™ Buffer

- Sterile water

 

Quality control

- Cloning efficiency: 99%

 

Standard reaction conditions

6X TOPcloner™ Buffer

1 μl

pTOP TA-Blunt V2 (10 ng/μl)

1 μl

PCR product

1~4 μl

Sterile water

up to 6 μl

Incubate at room temperature(25) for 5 min

Transformation

● Material Safety Data Sheet

- Enzynomics_MSDS_EZ017_TOPcloner™ TA-Blunt core Kit_E


● Vector Information

1. Vector full sequence

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