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EZ-Fusion™ Cloning Kit

No Product Name Cat.# Conc Size Price(₩) Add
1 EZ-Fusion™ Cloning Kit EZ015S - 10 rxns ₩ 195,000 추가
2 EZ-Fusion™ Cloning Kit EZ015M - 20 rxns ₩ 351,000 추가
3 EZ-Fusion™ Cloning Kit EZ015L - 40 rxns ₩ 663,000 추가
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EZ-Fusion™ Cloning kit principle video

▶ EZ-Fusion™ Cloning kit primer design program


Product description

EZ-Fusion™ Cloning Kit is designed for rapid and efficient cloning of PCR-amplified DNA fragments in to any cloning vectors including commercial and customized ones. It is also possible to insert one or more DNA fragments into a cloning vector in a defined orientation. EZ-Fusion™ Cloning PreMIX allows terminal 10 to 20 base pair overlapping homologous DNA at the ends of linearized vectors (usually by restriction enzymes) and insert DNA fragments (usually PCR-amplified) to precisely recombine to generate cloning products. In addition, the EZ-Fusion™ Cloning Kit can be used to clone long DNA fragments with high efficiency.

 

Characteristics

- Sub-cloning is unnecessary

- Highly efficient

- Seamless construction

- Flexibility to clone single or multiple fragments

- Can be used to perform site-directed mutagenesis

 

Applications

- PCR cloning

- HTP cloning

- Multiple fragment cloning

- Gene synthesis

- Gene design

- Mutagenesis

- Domain swapping

- Domain modification

 

Quality control

- Cloning efficiency: 99%

 

Kit contents

- 5X EZ-Fusion™ Cloning PreMIX

- pUC19 Control Vector, linearized (50 ng/µl)

- 2 kb Control Insert (40 ng/µl)

- Sterile water

- DH5α Chemically Competent E. coli 

 

 

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