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Real-time PCR Reagents

Home > PRODUCTS > Real-time PCR Reagents

RbTaq™ qPCR 2X PreMIX
(TaqMan Probe) new

No Product Name Cat.# Conc Size Price(₩) Add
1 RbTaq™ qPCR 2X PreMIX
(TaqMan Probe)
RT630S 2X 200 reactions ₩ 120,000 추가
2 RbTaq™ qPCR 2X PreMIX
(TaqMan Probe)
RT630M 2X 500 reactions ₩ 240,000 추가
Product Name Cat.# Size Price(₩) EA Total(₩) 삭제
Total : 장바구니에 담기 바로 구매 하기
plus minus 삭제

 ● Product Selection Guide by qPCR instruments



Product description

RbTaq™ qPCR 2X PreMIX (TaqMan Probe) is a preassembled liquid mixture that contains hot-start Taq, optimal reaction buffer, dNTP and stabilizing agents except primers, probes, and template. RbTaq™ qPCR 2X PreMIX (TaqMan Probe) is a repebody (Rb) mediated hot start method of Taq DNA polymerase. In the hot start method, Taq DNA polymerse is combined with a repebody that specifically binds to the enzyme and inactivates until the first denaturation step. Thus, RbTaq ™ qPCR 2X PreMIX (TaqMan Probe) is suitable for high-speed PCR and designed to have enhanced specificity, sensitivity and amplification efficiency of various targets.

 

Characteristics

- High specificity: Enhanced specificity by the use of Hot Start PCR polymerase

- High-speed: Repebody mediated hot start Taq allows rapid and accurate qPCR

- Stability: Enhanced stability allows one year storage at -20

- Easy-to-use: PreMIX contains everything needed except for primers, template and probe

- High reproducibility is ensured by rigorous quality control

- Included 100 nM ROX reference Dye

 

Applications

- Real-time quantification of gDNA and cDNA targets

- Gene expression profiling

- Microbial & viral pathogen detection

 

Standard reaction conditions

- PCR mixture

RbTaq™ qPCR 2X PreMIX (TaqMan Probe)

10 μl

Template DNA

1 μl

Probe (2~4 pmol/μl)

1 μl

Primers 1 (5~10 pmol/μl)

1 μl

Primers 2 (5~10 pmol/μl)

1 μl

Sterile water (RNase free)

up to 20 μl

 

- PCR cycle

Initial denaturationa

95

3 min

Denaturation

95

≤ 10 sec

Annealing & Elongation

60

≤ 60 sec

Number of cycles

35~40 times

a 30 sec at 95 for cDNA and 3 min at 95 for genomic DNA is recommended for enzyme activation.

 

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