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Real-time PCR Reagents

Home > PRODUCTS > Real-time PCR Reagents

RbTaq™ Fast qPCR 2X PreMIX
(SYBR Green with low ROX) new

No Product Name Cat.# Conc Size Price(₩) Add
1 RbTaq™ Fast qPCR 2X PreMIX
(SYBR Green with low ROX)
RT540S 2X 200 reactions ₩ 140,000 추가
2 RbTaq™ Fast qPCR 2X PreMIX
(SYBR Green with low ROX)
RT540M 2X 500 reactions ₩ 280,000 추가
Product Name Cat.# Size Price(₩) EA Total(₩) 삭제
Total : 장바구니에 담기 바로 구매 하기
plus minus 삭제

 ● Product Selection Guide by qPCR instruments



Product description

RbTaq™ Fast qPCR 2X PreMIX (SYBR Green with low ROX) is a preassembled liquid mixture that contains genetically engineered high-speed hot-start Taq, optimal reaction buffer, dNTP, stabilizing agents, ROX reference dye and SYBR Green I dye. RbTaq™ Fast qPCR 2X PreMIX (SYBR Green with low ROX) is a repebody (Rb) mediated hot start method of Taq DNA polymerase. In the hot start method, engineered Taq DNA polymerse is combined with a repebody that specifically binds to the enzyme and inactivates until the first denaturation step. Thus, RbTaq™ Fast qPCR 2X PreMIX (SYBR Green with low ROX) is suitable for fast PCR and designed to have enhanced specificity, sensitivity and amplification efficiency of various targets with the highest fluorescence signals. 

 

Characteristics

- Fast and High performance qPCR: Genetically engineered Taq and optimized

buffer system enable to quickly and accurately detect in less than 40 mins

- Resistance to various PCR inhibitors

- Stability: Enhanced stability allows one year storage at -20

- Easy-to-use: RbTaq™ Fast qPCR 2X PreMIX (SYBR Green with low ROX)

contains everything needed except for primers, template

- High reproducibility is ensured by rigorous quality control

 

Applications

- Fast real-time quantification of gDNA and cDNA targets

- Gene expression profiling

- Microbial & viral pathogen detection

 

Standard reaction conditions

- PCR mixture

RbTaq™ Fast qPCR 2X PreMIX (SYBR Green with low ROX)

10 μl

Template DNA

1 μl

Primers 1 (5~10 pmol/μl)

1 μl

Primers 2 (5~10 pmol/μl)

1 μl

Sterile water (RNase free)

up to 20 μl

 

- PCR cycle

Initial denaturationa

95

3 min

Denaturation

95

 ≤10 sec

Annealing/extension

60

≤10 sec

Number of cycles

~35 times

a 30 sec at 95 for cDNA and 3 min at 95 for genomic DNA is recommended for enzyme activation.

 



Figure. Reduced PCR lead time (~40 min) with RbTaq Fast qPCR 2X PreMIX

196 bp of GAPDH gene was qPCR amplified with serial diluted human cDNA as templates in the condition of standard and fast PCR protocol.


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