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Real-time PCR Reagents

Home > PRODUCTS > Real-time PCR Reagents

RbTaq™ qPCR 2X PreMIX
(SYBR Green with low ROX) new

No Product Name Cat.# Conc Size Price(₩) Add
1 RbTaq™ qPCR 2X PreMIX
(SYBR Green with low ROX)
RT530S 2X 200 reactions ₩ 120,000 추가
2 RbTaq™ qPCR 2X PreMIX
(SYBR Green with low ROX)
RT530M 2X 500 reactions ₩ 240,000 추가
Product Name Cat.# Size Price(₩) EA Total(₩) 삭제
Total : 장바구니에 담기 바로 구매 하기
plus minus 삭제

 ● Product Selection Guide by qPCR instruments



Product description

RbTaq™ qPCR 2X PreMIX (SYBR Green with low ROX) is a preassembled liquid mixture that contains hot-start Taq, optimal reaction buffer, dNTP, stabilizing agents, ROX reference dye and SYBR Green I dye. RbTaq™ qPCR 2X PreMIX (SYBR Green with low ROX) is a repebody (Rb) mediated hot start method of Taq DNA polymerase. In the hot start method, Taq DNA polymerse is combined with a repebody that specifically binds to the enzyme and inactivates until the first denaturation step. Thus, RbTaq ™ qPCR 2X PreMIX (SYBR Green with low ROX) is suitable for high-speed PCR and designed to have enhanced specificity, sensitivity and amplification efficiency of various targets.

 

Characteristics

- High specificity: Enhanced specificity by the use of Hot Start PCR polymerase

- High-speed: Repebody mediated hot start Taq allows rapid and accurate qPCR

- Stability: Enhanced stability allows one year storage at -20

- Easy-to-use: PreMIX contains everything needed except for primers, template and probe

- High reproducibility is ensured by rigorous quality control

 

Applications

- Real-time quantification of gDNA and cDNA targets

- Gene expression profiling

- Microbial & viral pathogen detection

 

Standard reaction conditions

- PCR mixture

RbTaq™ qPCR 2X PreMIX (SYBR Green with low ROX)

10 μl

Template DNA

1 μl

Primers 1 (5~10 pmol/μl)

1 μl

Primers 2 (5~10 pmol/μl)

1 μl

Sterile water (RNase free)

up to 20 μl

 

- PCR cycle

Initial denaturationa

95

3 min

Denaturation

95

 ≤10 sec

Annealing

60

15 sec

Elongation

72

15~30 sec

Number of cycles

35~40 times

a 30 sec at 95 for cDNA and 3 min at 95 for genomic DNA is recommended for enzyme activation.

 



Figure. 157 bp of GAPDH region was amplified using three different qPCR PreMIX (Enzynomics, Company T, and B) with human cDNA as a template. RbTaq qPCR 2X PreMIX (SYBR Green with low ROX) generated the fastest Ct value among them.

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