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Real-time PCR Reagents

Home > PRODUCTS > Real-time PCR Reagents

TOPreal™ qPCR 2X PreMIX
(SYBR Green with low ROX, UDG plus)

No Product Name Cat.# Conc Size Price(₩) Add
1 TOPreal™ qPCR 2X PreMIX
(SYBR Green with low ROX, UDG plus)
RT500US 2X 200 reactions ₩ 120,000 추가
2 TOPreal™ qPCR 2X PreMIX
(SYBR Green with low ROX, UDG plus)
RT500UM 2X 500 reaction ₩ 240,000 추가
Product Name Cat.# Size Price(₩) EA Total(₩) 삭제
Total : 장바구니에 담기 바로 구매 하기
plus minus 삭제

 ● Product Selection Guide by qPCR instruments



Product description

TOPreal™ qPCR 2X PreMIX (SYBR Green with low ROX, UDG plus) is a preassembled liquid mixture that contains nTaq-HOT DNA Polymerase (Cat. P725) optimal reaction buffer, dNTP, stabilizing agents, ROX reference dye and SYBR Green I dye. TOPreal™ qPCR 2X PreMIX (SYBR Green with low ROX, UDG plus) is formulated to have enhanced specificity, thereby minimizing nonspecific noise signal due to formation of primer dimers or non-specific products. Also, UDG and dUTP are included in the mixture to prevent the reamplification of carryover PCR product between reactions.

 

Characteristics

- Carry-over contamination control: contains UDG

- High specificity: Enhanced specificity by the use of Hot Start PCR

- Stability: Enhanced stability allows one year storage at -20°C.

- Easy-to-use: TOPreal™ qPCR 2X PreMIX (SYBR Green with low ROX, UDG plus)

  contains everything needed except for primers and template.

- High reproducibility ensured by rigorous quality control

 

Applications

- Real-time quantification of gDNA and cDNA targets

- Gene expression profiling

- Microbial & viral pathogen detection

 

Quality control

- Endonuclease assay

 

Standard reaction conditions

- PCR mixture

TOPreal™ qPCR 2X PreMIX (SYBR Green with low ROX, UDG plus)

10 μl

Template DNA

1 μl

Primers 1 (5~10 pmol/μl)

1 μl

Primers 2 (5~10 pmol/μl)

1 μl

Sterile water (RNase free)

up to 20 μl

 

- PCR cycle

Pre-incubation (for UDG)

50

4 min

Initial denaturationa

95

10~15 min

Denaturation

95

10 sec

Annealing

60

15 sec

Elongation

72

15~30 sec

Number of cycles

35~55 times

aAt least 10 min of initial denaturation time is required to fully activate the chemically modified PCR DNA polymerase

 

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