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Reverse Transcriptases

Home > PRODUCTS > Reverse Transcriptases

TOPscript™ One-step RT PCR DryMIX

No Product Name Cat.# Conc Size Price(₩) Add
1 TOPscript™ One-step RT PCR DryMIX
(20 μl reactions)
RT411 - 96 tubes ₩ 220,000 추가
2 TOPscript™ One-step RT PCR DryMIX
(20 μl reactions)
RT412 - 480 tubes ₩ 880,000 추가
Product Name Cat.# Size Price(₩) EA Total(₩) 삭제
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Product description

This product is TOPscript™ One-step RT-PCR DryMIX that is aliquoted into PCR tube strips. For example, 8 strips of 12 tubes are provided for 96 PCR reactions. TOPscript™ One-step RT PCR DryMIX is a ready-to-use mixtures containing dried components required for RT-PCR in one tube. For reactions, simply add template RNA and a pair of primers resuspended in water.

 

Characteristics

- Single-tube convenience

- Well-suited to analysis of a large number of samples.

- Synthesis of long cDNA

- Excellent sensitivity

 

Applications

- Virus detection

- Single-cell RT-PCR

- Gene-expression analysis

- cDNA library construction

- 3’ and 5’ RACE, RT-PCR

 

Components

- Reverse Transcriptase

- Hot-start Taq DNA Polymerase

- dNTP mixture

- One-step RT PCR reaction buffer

- Stabilizer

 

Storage

Stable up to 1 years at -20 (Storage at -20 is recommended).

 

Standard reaction conditions

- a)RT-PCR mixture

TOPscript™ One-step RT PCR DryMIX

1 tube

Template (total RNA, 10 ng ~ 1 μg/μl)

X μl

Gene specific primers (2~20 pmol/μl)

1 μl

Sterile water

up to 20 μl

aAssemble the reaction mixture on ice

 

- RT-PCR cycle

Reverse Transcription

50~55

30 min

PCR reaction

Initial denaturation

95

10 min

Denaturation

95

5 sec

b)Annealing

55~65

30~60 sec

Annealing & Elongation

60

30 sec

Number of cycles

30~45 times

When cycles are over, keep the reaction mixture at 4; may add 10 mM EDTA until use to prevent DNA degradation.

b)Recommended annealing temperatures is 5 to 10 below the lower Tm of the two primers used.

 


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