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Reverse Transcriptases

Home > PRODUCTS > Reverse Transcriptases

TOPscript™ RT DryMIX(dT18/dN6 plus)

No Product Name Cat.# Conc Size Price(₩) Add
1 TOPscript™ RT DryMIX (dT18/dN6 plus) RT220 - 96 tubes ₩ 270,000 추가
2 TOPscript™ RT DryMIX (dT18/dN6 plus) RT221 - 480 tubes ₩ 1,080,000 추가
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Product description

TOPscript™ RT DryMIX(dT18/dN6 plus) is formulated for maximal stability of TOPscript™ Reverse Transcriptase that is dried with reaction buffer, dNTP mixture, stabilizer, oligo dT primer and random hexamer primer. This product is ready to use upon the addition of template RNA in distilled water to each tube. TOPscript™ Reverse Transcriptase is genetically engineered version of M-MLV RT which is highly thermostable, thus can synthesize cDNA at elevated temperatures up to 60. This property is very useful when RNA templates are long and have extensive secondary structures. TOPscript™ Reverse Transcriptase is capable of synthesizing cDNA longer than 20 kb from messenger RNA.

 

Characteristics

- Simplicity: The dried pellet in TOPscript™ RT DryMIX is readily soluble upon addition of

template RNA in water.

- Handling: Well-suited to analysis of a large number of samples.

- Stability: The presence of a unique stabilizer ensures great stability of TOPscript™ Reverse Transcriptase.

- Synthesis of long cDNA

- Excellent sensitivity

 

Applications

- Synthesize first-strand cDNA,

- Array labeling

- cDNA libraries

- 3’ and 5’ RACE, RT-PCR

- Primer extension

 

Components

- TOPscript™ Reverse Transcriptase

- TOPscript™ RT buffer

- dNTP mixture

- RNase inhibitor

- Stabilizer

- Oligo (dT)18 primer

- Random hexamer

 

Storage buffer

20 mM Tris-Cl (pH7.5), 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.01% NP40, 50% glycerol

 

Standard reaction conditions

TOPscript™ RT DryMIX (dT18/dN6 plus)

1 tube

a)Template RNA

X μl

Sterile water (RNase free)

up to 20 μl

a)Prepare one of the following RNA template.

    - Total RNA: 1 ng~5 μg

    - Messenger RNA (mRNA): 1 ng~250 ng

    - Specific RNA: 0.01 pg~0.5 μg

→ Dissolve the dried pellet by vortexing and briefly spin down.

→ An additional annealing step is recommended:

    - if using oligo(dT)18, incubate at 42 for 5 min.

    - if using random hexamer, incubate at 25 for 10 min.

→ Incubate at 42~60 for 60 min.

→ Incubate at 95 for 5 min to inactivate the reaction.

 

Note

TOPscript™ Reverse Transcriptase performs optionally over the full range of 42~60. Typically, 50 is a good starting point. For RNAs containing secondary structure and other challenging targets, a synthesis temperature of 60 may be used without loss of performance.

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