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Polymerases

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2X RbTaq™ DyeMIX-HOT new

No Product Name Cat.# Conc Size Price(₩) Add
1 2X RbTaq™ DyeMIX-HOT P510HRB 2X 1 ml ₩ 70,000 추가
2 2X RbTaq™ DyeMIX-HOT P525HRB 2X 2.5 ml ₩ 140,000 추가
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Product description

2X RbTaq™ DyeMIX-HOT is similarly formulated to the 2X RbTaq™ PreMIX-HOT except that it contains loading dyes for further convenience of use. Thus, the reaction mixtures after PCR cycles are ready for agarose gel electrophoresis. This product includes RbTaq™ HOT (Cat. P725RB), and is optimized to increase the specificity and efficiency of target DNA amplification

 

Characteristics

- 2-Dye system: Easy for gel electrophoresis (xylene cyanol and Orange G)

- A-tail formation at 3’ ends of amplified duplex DNA

 

Applications

- High specific amplification of DNA fragments shorter than 3 kb.

- Amplification of cDNA and genomic DNA.

- Amplification of template DNA with secondary or higher ordered structure that is resistant to PCR amplification

- Primer extension

- Well-suited for an automated PCR machines, for which PCR reaction mixtures are prepared at room 

  temperature

- Colony PCR

 

Components(2X)

- RbTaq™ HOT DNA Polymerase: 0.2 unit/μl

- RbTaq™ HOT Buffer (containing 4 mM Mg2+)

- dNTP mixture: 0.4 mM each

- Stabilizer

- Dyes: Xylene cyanol and Orange G

 

Storage

Stable up to 18 months at -20 or 3 months at 4 (Storage at -20 is recommended).

 

Standard reaction conditions

- PCR mixture

2X RbTaq DyeMIX-HOT

10 μl

Template DNAa (0.1~500 ng/μl)

1 μl

Primer 1 (5 pmole/μl)

1 μl

Primer 2 (5 pmole/μl)

1 μl

Sterile water

up to 20 μl

aPlasmid DNA, 0.1 ng-30 ng; genomic DNA, 50 ng-500 ng

 

- PCR cycles

Step

Temperature

Time

Cycle

Initial denaturationa

95

3 min

1

Denaturation

95

30 sec

25~35

Annealingb

55~65

30~60 sec

Elongation

72

1 min/kb

Final elongation

72

5 min

1

When cycles are over, keep the reaction at 4; may add 10 mM EDTA until use to prevent DNA degradation

a30 sec at 95 for cDNA and 3 min at 95 for genomic DNA is recommended for enzyme activation.

bRecommend annealing temperature is 5 to 10 below the lower Tm of the two primers used.

 

● Material Safety Data Sheet

Enzynomics_MSDS_P510HRB_2X RbTaq™ DyeMIX-HOT

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